21.05.2012

General Meeting: People: Agata, Santiago, Thomas, Varsha, Karen, Maryam, Ali
Advisors/Supervisors: Ralf, Michael, Dominik, Svea
What do we have to do now? Ans=Divide the team for the individual parts:

Who is dealing with the homepage? Santiago, Agata

Who is dealing with the video? Ali + friends, Varsha (Shruti knows about video, they have a good camera), Thomas

Who takes care about the lab work?

• Arrange a meeting with Dominik and Alexander (Agata will write to them this week!)
• Define the project idea

Who wants to work with lipids? (Agata)

Who works with caDNAno? And DNA Origami + FRET + Aptamer System (Santiago, Maryam)

We want to ask Dominik + Alexander for papers about the theory behind the topic (maybe special papers) -> so everybody is at the same theoretical level: Everybody has to read it!! – short circle ten minutes presentations of the landmark papers

Who wants to work with the transcription system? Detection system for the transcription? (Karen, Varsha, Praveen) -> Contact person Michael Schlierf (T7-Polymerase)

Tomorrow:

Discuss about the Long Night of science? (7. of July)

• Who wants to do which poster? (do a little scetch) Competition, Applications DNA Nanotechnology, Our project + workshop for kids: how to fold DNA Origami^^
• Write in two languages a short abstract for tomorrow
• Write a general text to the people who want to support us
• Think of possible video ideas today (discuss them tomorrow)

Meeting every Monday 2 pm Meeting tomorrow 5:30 pm Meeting with supervisors:

We should ask Petra to get more PhD students from her as advisors (maybe go to their group meeting, present the idea, the competition and ask if somebody is interested to further support us.

Money + Funding:

• 2 pages of summary + write down all the expenses (include a figure in the project description)
• Contact Susann Fischer at DZNE!
• As soon as we have the description: supervisors could maybe contact some companies
• For lab space: we need a clear project description to contact other group leaders to get lab space
• Sum up the costs: we should write to get free access to confocal and Electron Microscopy at Technology Plattform of Biotec
• Maybe… Lab space in the Schwille lab (for vesicles) or B-cube (for FRET things or PCR machines)
• Short scetch about the project now: DNA Origami + lipids + transcription
• Include Dominik + Alexander in the mailing list (biomod-pr). Ask alexander for a short presentation
• If we go for the dimerization we can do cross-correlation to see that they get together
• Where should it happen? Maybe in GUVs it´s simpler,
• Alexander is a student worker in Ralf´s group and designs DNA Origami
• Aptamer guy: 1000 papers…. Itamar Willner, Friedrich Simmel about transcriptional oscillator
• Crucial point: how to get the switch between aptamer binding / conformational change to get the transcription to work.
• Getting DNA through membrane is difficult.
• Be clear about motivation to have a consistent story.
• In B-cube they can build 100 and 200 nm vesicles. Build up an artificial integrin system. Connect two vesicles…-> artificial cell adhesion system, vesicle fusion system: Michael knows…
• Find a natural system to mimic…. .