14.05.2012

Meeting at 1 pm People: Ali, Agata, Santiago, Mari, Thomas Projects List:

• Artificial Transcription System Membrane Bound:
  • Three supervisors involved in the project: Ralf, Erik, Michael (Petra)
  • Interesting and feasible project: coupling of Origami to membrane is very new and possible
  • Possible to split into parts: DNA Origami coupling to membrane: Artificial transcription cascade: Bring the Origami at the membrane together
  • Techniques used: Formation of lipid bilayer, FRET, DNA Origami, Selection of Aptamers/complementary strand system, assays for DNA transcription.
  • Doing Origami inside of the cell.
  • Does it fit into BioMod? Because it is not really an architecture … but already an application.
  • What about the environmental conditions? The GUVs need exact conditions: a slight change in pH can disassemble the GUVs
  • In solution FRET should be no problem
  • Who would help? Possible supervisors: Alexander from Schwille lab, Dominik for DNA Origami
  • T7-Polymerase: evaluation of the artificial transcription system: Svea? Sarah?
  • Already established coupling of GUVs to DNA Origami
  • Costs? Origami costs something if we have a special design; Cholesterol costs sth…
  • Could be possible to buy them in B-cube (might be cheaper); buy one couple it to different sites (hybridize the single one)
  • Add fluorescent labels to staples is cost-effective…
  • Facility usage: Write potential times up, so we can use it in BioTec without paying to much
  • Points:
    • Coupling of DNA Origami to membrane
    • Origami hybridization
    • Find a correct aptamer
    • Detection of transcription
• Nanorockets:
  • IFW is not really coupled to Biotec.
  • Only 3 months
  • We would not have lab space
  • We don´t have a real project so far.
  • Supervisors only from that place
  • Techniques: Roll up, chemical vapor deposition, photoresist, layer deposition techniques, protein assays
  • Deposition of proteins into the tube
  • Problem to transfer the protocols from one lab to another
  • Difference in dimensions could be difficult
  • All measurements would have to be done on single rockets
• Microtubule capping:
  • Not splittable ( a bit): Build basket with antibody which could bind to the end of mts
  • Binding of several antibodies to the end; maybe is not defined cutted end, but different length of the filaments…
  • Many conceptual questions….
  • Good Expertise with Microtubules in Dresden (Stefan Diez, Howart)
  • Techniques: DNA Origami, FRET, Surface deposition,
  • Many question marks
  • Nucleate sth. from a DNA Origami; Create oriented arrays of MT;
• Signal amplification with Boxes:
  • How tight are the boxes?
  • Boxes itself already exist, not very new
  • How do we get molecules inside
  • Get the box should not be soo difficult: Use the published design: the staples
  • Amplification system which opens and activates other little robots: Like the Paper of the DNA Nanorobot…
  • Techniques?
• DNA Transformers:
  • Much simpler structure needed (than a car…)
  • We would need lots of TEM capacity
  • Could be possible
• Goal to the end of month: Who is doing what, which deadlines do we want to have. Everything needs to be theoretically resolved. Recipe for everything.
• Long Night of Science: DNA Origami for dummies:
• Workshop-> Applications for DNA Nanotechnology: Robots, Rockets, advertising for BioMod competition, create a platform for donation.
• Say what BioMod is,explain also transcription.
• Poster Our project explanation; BioMod; DNA Applications in Nanotechnology: 3 posters
• Something for kids: building some Origami animals or DNA double helix origami
• Meeting with supervisors at 14.05.2012:
  • Vote among ourselves:
  • Which project would be the coolest?
  • Which project would be the most reasonable?