Summary
- The biotin labelled oligos arrived.
- The aptamers and complements arrived.
- The labelling was done till today as the biotin labelled oligos arrived till wednesday
- Ask Yixin Zhang about the cholesterol labelled oligos->How does it work? could we do it? also discuss TEG spacer with him.
REMARKS/COMMENTS:
- Option given by svea->Polystyrene beads (1um) if QDs don't work.
- Keep in mid Articles in German for enterprises.
IMPORTANT TASKS:
To order (Everybody should be aware):
- Catcher complementary + chol @ 3'->To connect LUVs
- Anchor complementary + chol @ 3'->To connect the DNA origami with the GUVs IMPORTANT!!!!!
To do:
- Clarify the procedure for chol labelled oligos and the TEG spacer -> Yixin Zhang Cost and time are key!!!! (Praveen)
- Talk with the technician (or someone) that know about the process. To be done on monday. (Praveen)
- Get PDGF. Santiago (me) will insit with MP Biomedicals. But please-> Look for other companies and harrass them if it's necessary!!(Ali and Maryam)
- Start aptamer experiments right away. Characterize aptamer and test the locks->Does labelling affect/change function? (Ali and Maryam)
- Do GUV-QD exps. during this week-> Attach complementary stands to GUVs and QDs and do microscopy (Varsha, Praveen)
- Assemble new constructs and do Origami-QD exps-> Gel shift and TEM (Alex, Santiago)
- Advance with the wiki!! -> Define a solid layout (Santiago)
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