Origami (Alex):
- Cost 1160 euros, 7560 bp.
- Origami descrition see presentation and dropbox for extra details
- Question: Why the locks are not symmetric
- Effective radio of strands in the origami: 2.25nm, extra 0.25nm given by possible repulsion between strands.
- 45nmx37.35x40.5-44nm
- Two versions->guided, not guided
- Edge staples. Possible correction issues.
- Versions tried->Open shell, closed shell
- Separation by gel extraction->Doesn’t it damage the structure???
- IDEA: PCR Purification to separate single oligos from structure.
- Staining->which technique use Douglas for staining?
- Different protocols for staining 3 drops of water, 1 drop. Which one is the best??
- Is DNA interacting with the grid?? Maybe as it is laying It can change its structure and affect the results. Check with Douglas paper.
- Results->Good: Distinguishable regular spots, Bad: No differentiation between open and closed versions
- Errors in staining->probably during centrifugation and taking too much time.
- We should do a gel with different buffers, i.e, different concentrations of Mg(2+).
- Probable clash between Mg concentrations for DNA and GUV experiments.
- Maybe adding long catcher strands could help the structure to stay open.
- Schedule staining session with Suzanne Kretschmar and TEM session with Thomas Kurth.
- Question: How is DNA labeled?? Could we use QDots??
- Origami->Santiago has to be aware of every single detail of the DNA origami, know the sequences, structure of the origami, length, locks, guide strands.
- Gockan designed the webpage for I-GEM. He knows a lot about javascripting and Photoshop. Contact him!! Use I-Gem web pages as a measuring stick for webpage. BIOMOD ones are not that good.
- Who’s gonna do the finances? -> Varsha
- Tell to juliane, avoid any confusion for her.
- Problem-> Still clustering.
- Possible solutions-> Change buffer concentration and length of the linker
- LSM meeting tomorrow at 10:00->Praveen, Varsha and Mari are attending
- Aptamer group-> Ali should check the kinetic coefficients and know them by heart, not just saying that they’re on the paper.
- We have to know things to order from the sponsorship of the companies-> we have to do it fast; if it’s too late companies could be reluctant to give them.
- Start Downloading the pictures made with the LSM for safety issues
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